Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA kit (E08N0010)

BlueGene Biotech Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA kit (E08N0010)

E08N0010 Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA kit

The Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA kit can be used to identify samples from the Canine species. Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox can also be called MOX1, GP91-2, NOH1, NADPH Oxidase 1, Mitogenic Oxidase(Pyridine Nucleotide-Dependent Superoxide-Generating, NADH/NADPH mitogenic oxidase subunit P65-MOX, NADHPox.

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Specifications of Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA kit

Product Information
Cat. No.	E08N0010
Product Name	Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA kit
Species	Canine
Product Size	48 Tests / 96 Tests
Concentration	0.5-10ng/mL
Sensitivity	0.1ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5.0 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

NADHPox ELISA kit uses an anti-NADHPox antibody and an NADHPox-HRP conjugate in a competitive enzyme immunoassay method. NADHPox-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NADHPox antibody binding site between NADHPox from samples and NADHPox-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NADHPox. Since the number of sites is limited, as more sites are occupied by NADHPox from the sample, fewer sites are left to bind NADHPox-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NADHPox concentration in each sample is interpolated from this standard curve.

Quality Control on Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between NADHPox and analogues was observed.

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Summary of the Assay Procedure for Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA kit

Citations of Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA kit

E08N0010 has been referenced in the below publications：

Investigation of the mechanism underlying the antihypertensive effect of catheter-based radiofrequency renal sympathetic denervation in hypertensive dogs.